Supervisors: Dr Mike MacDonald and Prof Angus Lamond
Proteins are one of the essential building blocks of the human body. They control all biological systems in a cell. Many proteins act independently, though the vast majority interact with others for proper biological activity. Characterisation of these protein-protein interactions is critical to understand protein function and ultimately, the biology of the cell.
This project aims to develop new and exciting technologies to allow in depth research into nuclear dynamics, in particular using Fluorescent Lifetime Imaging Microscopy (FLIM) to quantitatively study protein-protein interactions. The project will create a novel three-dimensional FLIM system by implementing new imaging technologies to establish current methods of FLIM in a multidimensional environment.
Single Plane Illumination Microscopy (SPIM), commonly termed ‘lightsheet microscopy’, is a microscopy modality that utilises a number of basic principles to minimize light dose, maximise three-dimensional spatial resolution when imaging a sample and increase imaging speed.
Adding to the current state of the art in Dundee in lightsheet imaging, this project will combine the two modalities of FLIM and SPIM to create a SPIM-FLIM microscope.
In addition to combining these two microscopy modalities, a new array of single photon detectors will be used in the acquisition of data in this project. This array of Single Photon Avalanche Diodes (SPADs) will enable the system to detect single photons for increased temporal resolution under 120ps.
To date the above have been implemented in the Biophotonics Research labs based in Ninewells Hospital, University of Dundee. Preliminary characterization of the system is underway with imaging of fixed samples having been performed on both plant and human cells. Future work will progress to three-dimensional, with the potential for four-dimensional data acquisition of living cells.
[November 2017] D2.5: Analysis of how chromatin compacts during cell division
[November 2017] D2.4: Measurement of chromatin condensation during mitosis
[December 2015] D2.2: Improved temporal resolution in FLIM-SPIM
Journal and Conference Papers and Posters
[September 2016] Light-sheet Fluorescence Lifetime Imaging. Oral presentation at PHOTON16, Leeds, UK, 7th September 2016.
[August 2016] Light-sheet Fluorescence Lifetime Imaging. Poster presented at the Biophotonic approaches: From molecules to living systems conference, Dundee, Scotland, 23rd August 2016.
[August 2016] Light-sheet Fluorescence Lifetime Imaging. Oral presention at the Biophotonic approaches: From molecules to living systems conference, Dundee, Scotland, 23rd August 2016.
August 2016] Polarised Light Sheet Tomography. Oral presentation at the Biophotonic approaches: From molecules to living systems conference, Dundee, Scotland, 22nd August 2016.
[June 2016] Light-sheet Fluorescence Lifetime Imaging. Poster presented at PHOTONEX SCOTLAND, Edinburgh, Scotland, 8th June 2016.
[May 2016] Polarised light sheet tomography, Sascha L. Reidt, Daniel J. O’Brien, Kenneth Wood, and Michael P. MacDonald. Optics Express Vol. 24, Issue 10, pp. 11239-11249 (2016). [OPEN ACCESS]