Supervisors: Dr Mike MacDonald and Prof Kees Weijer
The aim of this project is a step towards the better understanding of the gastrulation process in the developing chick embryo. A digitally scanning light sheet microscope is able to deliver images of the epiblast forming the primitive streak with high spatio-temporal resolution, which has led to new insights in the biochemical processes involved in coordinated cell movements. Nevertheless, these images are degraded by the diffused photons in the highly scattering sample, which limits penetration depth dramatically. Three methods are exploited in the frame of this project to improve depth information:
- A multi-wavelength polarised light sheet tomography system to filter differently scattered photons
- Beam shaping with a spatial light modulator to generate non-diffracting laser beams
- Multi-modal detection of ultrasound generated using short laser pulses by the photoacoustic effect
These methods will reduce the influence of diffraction and scattering and improve the quality of the final images. Finally, they will enable the study of deeper lying cell layers, namely hypoblast, as well as the ingression and migration of mesoderm cells in the developing chick embryo.
Journal and Conference Papers and Posters
[November 2017] Gaussian vs Bellel light sheets: performance analysis in live large sample imaging. Sascha L Reidt, Ricardo Bango da Cunha Correia, Antti Karjalainen, Manli Chuai, Michael P. MacDonald and Cornelis J. Weijer, in revision for Nat.Sci Rep.
[August 2017] Gaussian vs Bessel light sheets: performance analysis in live large sample imaging. Oral presentation at SPIE Nanoscience and Engineering, San Diego, USA, 6th August 2017.
[September 2016] Polarised light sheet tomography. Oral presentation at PHOTON16, Leeds, UK, 7th September 2016.
[September 2016] Characterisation of Bessel and Gaussian beam illumination modes on LSM imaging quality of early chick embryo development. Oral presentation at 3rd Light Sheet Fluorescence Microscopy International Conference (LSFM 2016), Sheffield, UK, 3rd September 2016.
[August 2016] Characterisation of Bessel and Gaussian beam illumination modes on LSM imaging quality of early chick embryo development. Poster presented at the Biophotonic approaches: From molecules to living systems conference, Dundee, Scotland, 23rd August 2016.
[August 2016] Polarised Light Sheet Tomography. Oral presentation at the Biophotonic approaches: From molecules to living systems conference, Dundee, Scotland, 22nd August 2016.
[May 2016] Polarised light sheet tomography, Sascha L. Reidt, Daniel J. O’Brien, Kenneth Wood, and Michael P. MacDonald. Optics Express Vol. 24, Issue 10, pp. 11239-11249 (2016). [OPEN ACCESS]